Journal: International Journal of Molecular Sciences
Article Title: Pigment Epithelium-Derived Factor (PEDF) Receptors Are Involved in Survival of Retinal Neurons
doi: 10.3390/ijms22010369
Figure Lengend Snippet: List of oligonucleotides used for PCR amplifications.
Article Snippet: After washing with TBS, cells were blocked with TBS/1% dimethyl sulfoxide/0.3% Triton X-100/3% BSA, 2 mg/mL human gamma globulin (Sigma-Aldrich) for 1 h, and incubated with antibodies directed to PEDF-R (mouse anti-ATGL, Cat# sc-365278; Santa Cruz) or LR (rabbit anti-laminin receptor, Cat# ABC934; Merck Millipore, Darmstadt, Germany) at 4 °C overnight.
Techniques: Sequencing, Amplification